Effects of Inhibitory Compounds Present in Lignocellulosic Biomass Hydrolysates on the Growth of Bacillus subtilis

This study evaluated the individual and combined effects of inhibitory compounds formed during pretreatment lignocellulosic biomass on growth Bacillus subtilis. Ten commonly present in hydrolysates were evaluated, which included sugar degradation products (furfural 5-hydroxymethylfurfural), acetic acid, seven phenolic derived from lignin (benzoic vanillin, vanillic ferulic p-coumaric 4-hydroxybenzoic syringaldehyde). For inhibitors, syringaldehyde showed most toxic effect, completely inhibiting strain at 0.1 g/L. In sequence, assays using mixtures a concentration 12.5% their IC50 value performed to evaluate effect inhibitors growth. These experiments planned according Plackett–Burman experimental design. Statistical analysis results revealed that mixture, benzoic acid furfural potent affecting B. contribute better understanding microbial performance Such knowledge is important advance development sustainable biomanufacturing processes this cultivated complex media produced biomass, supporting efficient bio-based